In the case of the resistance of acute promyelocytic leukemia to all-trans retinoic acid (ATRA) induction treatment, arsenic trioxide (ATO) has proven to be an effective second line therapy. ![]() NAC significantly reduced the cytotoxic effect of the combination of PTL plus Ara-C.Ĭonclusion: PTL can significantly increase the cytotoxic and proapoptotic effects of Ara-C in an HL-60 cell line and this may warrant further research to improve the efficacy of acute promyelocytic leukemia therapy. ![]() Interactions between the investigated compounds were mostly synergistic. ![]() PTL significantly increased the cytotoxic and proapoptotic effects of Ara-C in comparison to the cytostatic drug alone. We did not observe a similar influence of NAC in the case of Ara-C activity. Cytotoxic and proapototic actions of PTL depend on the level of free radicals, because the addition of N-acetyl- L-cysteine (NAC) to incubation significantly reduced the effect of PTL. Results: PTL dose-dependently increased the percentage of dead cells. Cells were analyzed using flow cytometry. ![]() Methods: Cytotoxic effects and apoptosis were examined using propidium iodide (PI) or PI/Annexin-V staining, respectively. Aim: Investigation of the influence of parthenolide (PTL) on cytarabine (Ara-C) action in an acute promyelocytic leukemia HL-60 cell line and the role of oxidative stress in its action.
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